VIGS Assays

VIGS Assays

Blog Article

Virus-induced gene silencing (VIGS) is a powerful method to study gene function in plants.Tobacco rattle virus (TRV)-based here VIGS vector is the most efficient VIGS vector so far.This method was originally developed by the Dinesh-Kumar's group (Liu et al., 2002).

Here, we describe a rapid and high efficient TRV-based VIGS method for knocking down genes in Nicotiana benthamiana.For synovex one grass TRV-based VIGS, Agrobacterium culture containing pTRV1 and Agrobacterium culture containing pTRV2 with plant target gene fragment are mixed and infiltrated into the lower leaves of plant.After 2-3 weeks post infiltration, plant target gene will be silenced.